Functions

Genomics Core Facility

DNA Sanger Sequencing (ABI 3130xl Genetic Analyzer)
Plasmid DNA and PCR products purification (Qiacube workstation and Qiagen kits)
RNA or DNA purification from different sources: cell lines, tissues, blood, FFPE samples…(Qiacube workstation and Qiagen kits)
Spectrophotometric nucleic acids quantitation (Nanodrop ND-1000 and ND-8000 spectrophotometers)
Fluorometric nucleic acids quantitation (Qubit 2.0 Fluorometer)
RNA integrity analysis (Agilent 2100 Bioanalyzer)
RNA reverse transcription
Absolute or relative Real-Time quantitative PCR (ABI 7900HT Fast Real Time PCR System)
Real-Time allelic discrimination PCR (ABI 7900HT Fast Real Time PCR System)
Human cell lines authentication (ABI 3130xl Genetic Analyzer)
Authentication of mouse cell lines
Detection of contamination of human cell lines with mouse cells
PCR based mouse genotyping
Detection of Helicobacter in mouse faeces by QPCR (ABI7900HT and QS7)
Titration of lentiviral vectors by QPCR (ABI7900HT and QS7)
Fragment analysis (ABI 3130XL) (SeqStudio) (Consult Service)
Design of oligonucleotides/probes for Sanger Sequencing, PCR and Real Time PCR (Consult Service)
Detection of mycoplasma contamination in RNA samples by QPCR (ABI7900HT and QS7)
Detection of mycoplasma contamination in cell culture supernatants by means of QPCR (ABI7900HT and QS7).