INVESTIGACIÓN

 
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PUBLICACIONES

 

 

spacerTrabajos Publicados por María Angeles Pajares Tarancón
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  1. Sánchez-Pérez, G.F.; Pajares, M.Á. "Polar Interactions at the Dimer-Dimer Interface of Methionine Adenosyltransferase MAT I Control Tetramerization.". Int J Mol Sci. 22(24): . (2021). (PMID: 34948004).
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  2. Portillo, F.; Vázquez, J.; Pajares, M.A. "Protein-protein interactions involving enzymes of the mammalian methionine and homocysteine metabolism.". Biochimie. (): . (2020). (PMID: 32105812).
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  3. Partearroyo, T.; Murillo-Cuesta, S.; Vallecillo, N.; Bermúdez-Muñoz, J.M.; Rodríguez-de la Rosa, L.; Mandruzzato, G.; Celaya, A.M.; Zeisel, S.H.; Pajares, M.A.; Varela-Moreiras, G.; Varela-Nieto, I. "Betaine-homocysteine S-methyltransferase deficiency causes increased susceptibility to noise-induced hearing loss associated with plasma hyperhomocysteinemia.". FASEB J.. 33(5): 5942-5956. (2019). (PMID: 30753104).
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  4. Garrido, F.; Pacheco, M.; Vargas-Martínez, R.; Velasco-García, R.; Jorge, I.; Serrano, H.; Portillo, F.; Vázquez, J.; Pajares, M.Á. "Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase.". PLoS ONE. 13(6): e0199472. (2018). (PMID: 29924862).
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  5. Pajares, M.; Cuadrado, A.; Engedal, N.; Jirsova, Z.; Cahova, M. "The Role of Free Radicals in Autophagy Regulation: Implications for Ageing.". Oxid Med Cell Longev. 2018(): 2450748. (2018). (PMID: 29682156).
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  6. Partearroyo, T.; Vallecillo, N.; Pajares, M.A.; Varela-Moreiras, G.; Varela-Nieto, I. "Cochlear Homocysteine Metabolism at the Crossroad of Nutrition and Sensorineural Hearing Loss.". Front Mol Neurosci. 10(): 107. (2017). (PMID: 28487633).
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  7. Pérez-Miguelsanz, J.; Vallecillo, N.; Garrido, F.; Reytor, E.; Pérez-Sala, D.; Pajares, M.A. "Betaine homocysteine S-methyltransferase emerges as a new player of the nuclear methionine cycle.". Biochim. Biophys. Acta. (): . (2017). (PMID: 28288879).
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  8. Sánchez-Gómez, F.J.; Díez-Dacal, B.; García-Martín, E.; Agúndez, J.A.; Pajares, M.A.; Pérez-Sala, D. "Detoxifying Enzymes at the Cross-Roads of Inflammation, Oxidative Stress, and Drug Hypersensitivity: Role of Glutathione Transferase P1-1 and Aldose Reductase.". Front Pharmacol. 7(): 237. (2016). (PMID: 27540362).
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  9. Martínez-Vega, R.; Murillo-Cuesta, S.; Partearroyo, T.; Varela-Moreiras, G.; Varela-Nieto, I.; Pajares, M.A. "Long-Term Dietary Folate Deficiency Accelerates Progressive Hearing Loss on CBA/Ca Mice.". Front Aging Neurosci. 8(): 209. (2016). (PMID: 27630560).
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  10. Pérez, C.; Pérez-Zúñiga, F.J.; Garrido, F.; Reytor, E.; Portillo, F.; Pajares, M.A. "The Oncogene PDRG1 Is an Interaction Target of Methionine Adenosyltransferases.". PLoS ONE. 11(8): e0161672. (2016). (PMID: 27548429).
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  11. Martínez-Vega, R.; Partearroyo, T.; Vallecillo, N.; Varela-Moreiras, G.; Pajares, M.A.; Varela-Nieto, I. "Long-term omega-3 fatty acid supplementation prevents expression changes in cochlear homocysteine metabolism and ameliorates progressive hearing loss in C57BL/6J mice.". J. Nutr. Biochem.. 26(12): 1424-1433. (2015). (PMID: 26321228).
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  12. Martínez-Vega, R.; Garrido, F.; Partearroyo, T.; Cediel, R.; Zeisel, S.H.; Martínez-Álvarez, C.; Varela-Moreiras, G.; Varela-Nieto, I.; Pajares, M.A. "Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism.". FASEB J.. 29(2): 418-432. (2015). (PMID: 25384423).
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  13. Delgado, M.; Garrido, F.; Pérez-Miguelsanz, J.; Pacheco, M.; Partearroyo, T.; Pérez-Sala, D.; Pajares, M.A. "Acute liver injury induces nucleocytoplasmic redistribution of hepatic methionine metabolism enzymes.". Antioxid. Redox Signal.. 20(16): 2541-2554. (2014). (PMID: 24124652).
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  14. Pajares, M.A.; Alvarez, L.; Pérez-Sala, D. "How are mammalian methionine adenosyltransferases regulated in the liver? A focus on redox stress.". FEBS Lett.. 587(12): 1711-1716. (2013). (PMID: 23669363).
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  15. Sánchez-Gómez, F.J.; Dorado, C.G.; Ayuso, P.; Agúndez, J.A.; Pajares, M.A.; Pérez-Sala, D. "Modulation of GSTP1-1 oligomerization by electrophilic inflammatory mediators and reactive drugs.". Inflamm Allergy Drug Targets. 12(3): 162-171. (2013). (PMID: 23596995).
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  16. González, B.; Garrido, F.; Ortega, R.; Martínez-Júlvez, M.; Revilla-Guarinos, A.; Pérez-Pertejo, Y.; Velázquez-Campoy, A.; Sanz-Aparicio, J.; Pajares, M.A. "NADP+ binding to the regulatory subunit of methionine adenosyltransferase II increases intersubunit binding affinity in the hetero-trimer.". PLoS ONE. 7(11): e50329. (2012). (PMID: 23189196).
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  17. Garrido, F.; Taylor, J.C.; Alfonso, C.; Markham, G.D.; Pajares, M.A. "Structural basis for the stability of a thermophilic methionine adenosyltransferase against guanidinium chloride.". Amino Acids. 42(1): 361-373. (2012). (PMID: 21132339).
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  18. Pajares, M.A.; Markham, G.D. "Methionine adenosyltransferase (s-adenosylmethionine synthetase).". Adv. Enzymol. Relat. Areas Mol. Biol.. 78(): 449-521. (2011). (PMID: 22220481).
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  19. Garrido, F.; Estrela, S.; Alves, C.; Sánchez-Pérez, G.F.; Sillero, A.; Pajares, M.A. "Refolding and characterization of methionine adenosyltransferase from Euglena gracilis.". Protein Expr. Purif.. 79(1): 128-136. (2011). (PMID: 21605677).
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  20. Sánchez-Gómez, F.J.; Díez-Dacal, B.; Pajares, M.A.; Llorca, O.; Pérez-Sala, D. "Cyclopentenone prostaglandins with dienone structure promote cross-linking of the chemoresistance-inducing enzyme glutathione transferase P1-1.". Mol. Pharmacol.. 78(4): 723-733. (2010). (PMID: 20631055).
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  21. Reytor, E.; Pérez-Miguelsanz, J.; Alvarez, L.; Pérez-Sala, D.; Pajares, M.A. "Conformational signals in the C-terminal domain of methionine adenosyltransferase I/III determine its nucleocytoplasmic distribution.". FASEB J.. 23(10): 3347-3360. (2009). (PMID: 19497982).
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  22. Garrido, F.; Alfonso, C.; Taylor, J.C.; Markham, G.D.; Pajares, M.A. "Subunit association as the stabilizing determinant for archaeal methionine adenosyltransferases.". Biochim. Biophys. Acta. 1794(7): 1082-1090. (2009). (PMID: 19348969).
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  23. Markham, G.D.; Pajares, M.A. "Structure-function relationships in methionine adenosyltransferases.". Cell. Mol. Life Sci.. 66(4): 636-648. (2009). (PMID: 18953685).
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  24. Delgado, M.; Pérez-Miguelsanz, J.; Garrido, F.; Rodríguez-Tarduchy, G.; Pérez-Sala, D.; Pajares, M.A. "Early effects of copper accumulation on methionine metabolism.". Cell. Mol. Life Sci.. 65(13): 2080-2090. (2008). (PMID: 18560753).
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  25. Pajares, M.A.; Pérez-Sala, D. "Betaine homocysteine S-methyltransferase: just a regulator of homocysteine metabolism?". Cell. Mol. Life Sci.. 63(23): 2792-2803. (2006). (PMID: 17086380).
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  26. Garrido, F.; Gasset, M.; Sanz-Aparicio, J.; Alfonso, C.; Pajares, M.A. "Rat liver betaine-homocysteine S-methyltransferase equilibrium unfolding: insights into intermediate structure through tryptophan substitutions.". Biochem J. 391(Pt 3): 589-599. (2005). (PMID: 15943585).
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  27. Iloro, I.; Chehín, R.; Goñi, F.M.; Pajares, M.A.; Arrondo, J.L. "Methionine adenosyltransferase alpha-helix structure unfolds at lower temperatures than beta-sheet: a 2D-IR study.". Biophys. J.. 86(6): 3951-3958. (2004). (PMID: 15189891).
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  28. González, B.; Pajares, M.A.; Martínez-Ripoll, M.; Blundell, T.L.; Sanz-Aparicio, J. "Crystal structure of rat liver betaine homocysteine s-methyltransferase reveals new oligomerization features and conformational changes upon substrate binding.". J. Mol. Biol.. 338(4): 771-782. (2004). (PMID: 15099744).
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  29. González-Iglesias, R.; Elvira, G.; Rodríguez-Navarro, J.A.; Vélez, M.; Calero, M.; Pajares, M.A.; Gasset, M. "Cu2+ binding triggers alphaBoPrP assembly into insoluble laminar polymers.". FEBS Lett.. 556(1-3): 161-166. (2004). (PMID: 14706844).
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  30. Sánchez-Pérez, G.F.; Bautista, J.M.; Pajares, M.A. "Methionine adenosyltransferase as a useful molecular systematics tool revealed by phylogenetic and structural analyses.". J. Mol. Biol.. 335(3): 693-706. (2004). (PMID: 14687567).
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  31. González, B.; Pajares, M.A.; Hermoso, J.A.; Guillerm, D.; Guillerm, G.; Sanz-Aparicio, J. "Crystal structures of methionine adenosyltransferase complexed with substrates and products reveal the methionine-ATP recognition and give insights into the catalytic mechanism.". J. Mol. Biol.. 331(2): 407-416. (2003). (PMID: 12888348).
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  32. González, B.; Campillo, N.; Garrido, F.; Gasset, M.; Sanz-Aparicio, J.; Pajares, M.A. "Active-site-mutagenesis study of rat liver betaine-homocysteine S-methyltransferase.". Biochem. J.. 370(Pt 3): 945-952. (2003). (PMID: 12487625).
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  33. Sanchez-Perez, G.F.; Gasset, M.; Calvete, J.J.; Pajares, M.A. "Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase.". J. Biol. Chem.. 278(9): 7285-7293. (2003). (PMID: 12496263).
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  34. Pérez-Pertejo, Y.; Reguera, R.M.; Villa, H.; García-Estrada, C.; Balaña-Fouce, R.; Pajares, M.A.; Ordóñez, D. "Leishmania donovani methionine adenosyltransferase. Role of cysteine residues in the recombinant enzyme.". Eur. J. Biochem.. 270(1): 28-35. (2003). (PMID: 12492472).
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  35. González, B.; Pajares, M.A.; Too, H.P.; Garrido, F.; Blundell, T.L.; Sanz-Aparicio, J. "Crystallization and preliminary X-ray study of recombinant betaine-homocysteine S-methyltransferase from rat liver.". Acta Crystallogr. D Biol. Crystallogr.. 58(Pt 9): 1507-1510. (2002). (PMID: 12198317).
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  36. González-Iglesias, R.; Pajares, M.A.; Ocal, C.; Espinosa, J.C.; Oesch, B.; Gasset, M. "Prion protein interaction with glycosaminoglycan occurs with the formation of oligomeric complexes stabilized by Cu(II) bridges.". J. Mol. Biol.. 319(2): 527-540. (2002). (PMID: 12051926).
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  37. Gasset, M.; Alfonso, C.; Neira, J.L.; Rivas, G.; Pajares, M.A. "Equilibrium unfolding studies of the rat liver methionine adenosyltransferase III, a dimeric enzyme with intersubunit active sites.". Biochem. J.. 361(Pt 2): 307-315. (2002). (PMID: 11772402).
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  38. López-Vara, M.C.; Gasset, M.; Pajares, M.A. "Refolding and characterization of rat liver methionine adenosyltransferase from Escherichia coli inclusion bodies.". Protein Expr. Purif.. 19(2): 219-226. (2000). (PMID: 10873534).
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  39. González, B.; Pajares, M.A.; Hermoso, J.A.; Alvarez, L.; Garrido, F.; Sufrin, J.R.; Sanz-Aparicio, J. "The crystal structure of tetrameric methionine adenosyltransferase from rat liver reveals the methionine-binding site.". J. Mol. Biol.. 300(2): 363-375. (2000). (PMID: 10873471).
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  40. Martínez-Chantar, M.L.; Pajares, M.A. "Assignment of a single disulfide bridge in rat liver methionine adenosyltransferase.". Eur. J. Biochem.. 267(1): 132-137. (2000). (PMID: 10601859).
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  41. Alvarez, L.; Sánchez-Góngora, E.; Mingorance, J.; Pajares, M.A.; Mato, J.M. "Characterization of rat liver-specific methionine adenosyltransferase gene promoter. Role of distal upstream cis-acting elements in the regulation of the transcriptional activity.". J. Biol. Chem.. 272(36): 22875-22883. (1997). (PMID: 9278450).
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  42. Mato, J.M.; Alvarez, L.; Ortiz, P.; Pajares, M.A. "S-adenosylmethionine synthesis: molecular mechanisms and clinical implications.". Pharmacol. Ther.. 73(3): 265-280. (1997). (PMID: 9175157).
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  43. Gil, B.; Pajares, M.A.; Mato, J.M.; Alvarez, L. "Glucocorticoid regulation of hepatic S-adenosylmethionine synthetase gene expression.". Endocrinology. 138(3): 1251-1258. (1997). (PMID: 9048633).
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  44. Mingorance, J.; Alvarez, L.; Pajares, M.A.; Mato, J.M. "Recombinant rat liver S-adenosyl-L-methionine synthetase tetramers and dimers are in equilibrium.". Int. J. Biochem. Cell Biol.. 29(3): 485-491. (1997). (PMID: 9202427).
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  45. Sánchez-Góngora, E.; Pastorino, J.G.; Alvarez, L.; Pajares, M.A.; García, C.; Viña, J.R.; Mato, J.M.; Farber, J.L. "Increased sensitivity to oxidative injury in chinese hamster ovary cells stably transfected with rat liver S-adenosylmethionine synthetase cDNA.". Biochem. J.. 319 ( Pt 3)(): 767-773. (1996). (PMID: 8920979).
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  46. Martínez-Chantar, M.L.; Pajares, M.A. "Role of thioltransferases on the modulation of rat liver S-adenosylmethionine synthetase activity by glutathione.". FEBS Lett.. 397(2-3): 293-297. (1996). (PMID: 8955366).
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  47. Gil, B.; Casado, M.; Pajares, M.A.; Boscá, L.; Mato, J.M.; Martín-Sanz, P.; Alvarez, L. "Differential expression pattern of S-adenosylmethionine synthetase isoenzymes during rat liver development.". Hepatology. 24(4): 876-881. (1996). (PMID: 8855191).
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  48. Gassó, M.; Rubio, M.; Varela, G.; Cabré, M.; Caballería, J.; Alonso, E.; Deulofem, R.; Camps, J.; Giménez, A.; Pajares, M.; Parés, A.; Mato, J.M.; Rodés, J. "Effects of S-adenosylmethionine on lipid peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis.". J. Hepatol.. 25(2): 200-205. (1996). (PMID: 8878782).
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  49. Mingorance, J.; Alvarez, L.; Sánchez-Góngora, E.; Mato, J.M.; Pajares, M.A. "Site-directed mutagenesis of rat liver S-adenosylmethionine synthetase. Identification of a cysteine residue critical for the oligomeric state.". Biochem. J.. 315 ( Pt 3)(): 761-766. (1996). (PMID: 8645155).
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  50. Deigner, H.P.; Mato, J.M.; Pajares, M.A. "Study of the rat liver S-adenosylmethionine synthetase active site with 8-azido ATP.". Biochem. J.. 308 ( Pt 2)(): 565-571. (1995). (PMID: 7772043).
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  51. Mato, J.M.; Alvarez, L.; Ortiz, P.; Mingorance, J.; Durán, C.; Pajares, M.A. "S-adenosyl-L-methionine synthetase and methionine metabolism deficiencies in cirrhosis.". Adv. Exp. Med. Biol.. 368(): 113-117. (1994). (PMID: 7741002).
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  52. Pajares, M.A.; Durán, C.; Corrales, F.; Mato, J.M. "Protein kinase C phosphorylation of rat liver S-adenosylmethionine synthetase: dissociation and production of an active monomer.". Biochem. J.. 303 ( Pt 3)(): 949-955. (1994). (PMID: 7980467).
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  53. Alvarez, L.; Mingorance, J.; Pajares, M.A.; Mato, J.M. "Expression of rat liver S-adenosylmethionine synthetase in Escherichia coli results in two active oligomeric forms.". Biochem. J.. 301 ( Pt 2)(): 557-561. (1994). (PMID: 8043003).
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  54. Corrales, F.; Giménez, A.; Alvarez, L.; Caballería, J.; Pajares, M.A.; Andreu, H.; Parés, A.; Mato, J.M.; Rodés, J. "S-adenosylmethionine treatment prevents carbon tetrachloride-induced S-adenosylmethionine synthetase inactivation and attenuates liver injury.". Hepatology. 16(4): 1022-1027. (1992). (PMID: 1398482).
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  55. Pajares, M.A.; Durán, C.; Corrales, F.; Pliego, M.M.; Mato, J.M. "Modulation of rat liver S-adenosylmethionine synthetase activity by glutathione.". J. Biol. Chem.. 267(25): 17598-17605. (1992). (PMID: 1517209).
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  56. Pajares, M.A.; Corrales, F.; Durán, C.; Mato, J.M.; Alvarez, L. "How is rat liver S-adenosylmethionine synthetase regulated?". FEBS Lett.. 309(1): 1-4. (1992). (PMID: 1511738).
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  57. Alvarez, L.; Asunción, M.; Corrales, F.; Pajares, M.A.; Mato, J.M. "Analysis of the 5' non-coding region of rat liver S-adenosylmethionine synthetase mRNA and comparison of the Mr deduced from the cDNA sequence and the purified enzyme.". FEBS Lett.. 290(1-2): 142-146. (1991). (PMID: 1915866).
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  58. Corrales, F.; Ochoa, P.; Rivas, C.; Martin-Lomas, M.; Mato, J.M.; Pajares, M.A. "Inhibition of glutathione synthesis in the liver leads to S-adenosyl-L-methionine synthetase reduction.". Hepatology. 14(3): 528-533. (1991). (PMID: 1874498).
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  59. Ganter, U.M.; Longstaff, C.; Pajares, M.A.; Rando, R.R.; Siebert, F. "Fourier transform infrared studies of active-site-methylated rhodopsin. Implications for chromophore-protein interaction, transducin activation, and the reaction pathway.". Biophys. J.. 59(3): 640-644. (1991). (PMID: 2049524).
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  60. Pajares, M.A.; Corrales, F.J.; Ochoa, P.; Mato, J.M. "The role of cysteine-150 in the structure and activity of rat liver S-adenosyl-L-methionine synthetase.". Biochem. J.. 274 ( Pt 1)(): 225-229. (1991). (PMID: 2001237).
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  61. Mato, J.M.; Corrales, F.; Martin-Duce, A.; Ortiz, P.; Pajares, M.A.; Cabrero, C. "Mechanisms and consequences of the impaired trans-sulphuration pathway in liver disease: Part I. Biochemical implications.". Drugs. 40 Suppl 3(): 58-64. (1990). (PMID: 2081481).
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  62. Corrales, F.; Cabrero, C.; Pajares, M.A.; Ortiz, P.; Martin-Duce, A.; Mato, J.M. "Inactivation and dissociation of S-adenosylmethionine synthetase by modification of sulfhydryl groups and its possible occurrence in cirrhosis.". Hepatology. 11(2): 216-222. (1990). (PMID: 2307400).
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  63. Nakamura, H.; Kishi, Y.; Pajares, M.A.; Rando, R.R. "Structural basis of protein kinase C activation by tumor promoters.". Proc. Natl. Acad. Sci. U.S.A.. 86(24): 9672-9676. (1989). (PMID: 2602368).
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  64. Fierro, J.F.; Pajares, M.A.; Hardisson, C. "Calcium-dependent binding between calmodulin and lysozyme.". FEBS Lett.. 247(1): 22-24. (1989). (PMID: 2707447).
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  65. Pajares, M.A.; Rando, R.R. "The active-site environment of rhodopsin.". J. Biol. Chem.. 264(12): 6804-6809. (1989). (PMID: 2523385).
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  66. Villalba, M.; Pajares, M.A.; Renart, M.F.; Mato, J.M. "Protein kinase C catalyses the phosphorylation and activation of rat liver phospholipid methyltransferase.". Biochem. J.. 241(3): 911-916. (1987). (PMID: 3593229).
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  67. Pajares, M.A.; Villalba, M.; Mato, J.M. "Purification of phospholipid methyltransferase from rat liver microsomal fraction.". Biochem. J.. 237(3): 699-705. (1986). (PMID: 3800912).
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  68. Villalba, M.; Varela, I.; Mérida, I.; Pajares, M.A.; Martínez del Pozo, A.; Mato, J.M. "Modulation by the ratio S-adenosylmethionine/S-adenosylhomocysteine of cyclic AMP-dependent phosphorylation of the 50 kDa protein of rat liver phospholipid methyltransferase.". Biochim. Biophys. Acta. 847(3): 273-279. (1985). (PMID: 2998482).
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  69. Pajares, M.A.; Alemany, S.; Varela, I.; Marin Cao, D.; Mato, J.M. "Purification and photoaffinity labelling of lipid methyltransferase from rat liver.". Biochem. J.. 223(1): 61-66. (1984). (PMID: 6497846).
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  70. Varela, I.; Mérida, I.; Pajares, M.; Villalba, M.; Mato, J.M. "Activation of partially purified rat liver lipid methyltransferase by phosphorylation.". Biochem. Biophys. Res. Commun.. 122(3): 1065-1070. (1984). (PMID: 6332623).
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